ABSTRACT
Ferroptosis is a form of iron-dependent regulated cell death. Evidence of its existence and the effects of its inhibitors on subarachnoid hemorrhage (SAH) is still lacking. In the present study, we found that liproxstatin-1 protected HT22 cells against hemin-induced injury by protecting mitochondrial functions and ameliorating lipid peroxidation. In in vivo experiments, we demonstrated the presence of characteristic shrunken mitochondria in ipsilateral cortical neurons after SAH. Moreover, liproxstatin-1 attenuated the neurological deficits and brain edema, reduced neuronal cell death, and restored the redox equilibrium after SAH. The inhibition of ferroptosis by liproxstatin-1 was associated with the preservation of glutathione peroxidase 4 and the downregulation of acyl-CoA synthetase long-chain family member 4 as well as cyclooxygenase 2. In addition, liproxstatin-1 decreased the activation of microglia and the release of IL-6, IL-1β, and TNF-α. These data enhance our understanding of cell death after SAH and shed light on future preclinical studies.
ABSTRACT
Objective This study aimed to evaluate the model of integrated drowning interventions for the floating children in Ningbo City.Methods A two -year program of integrated drowning intervention had been carried out in Ningbo City since 2013,and the floating children in Jiangbei District and Yinzhou District were selected as the experiment and control group respectively.Baseline and final survey was all conducted in these two groups.A total of 7 736 and 7730 students from 1 st -9 th grade in the Migrant Workers'Children Schools were recruited by multi -stage random sampling and surveyed by the self -reported questionnaires in these two surveys respectively.The unconditional logistic regression model was adopted mainly for the multivariate analysis.Results The incidence rate of non -fatal drowning for the floating children in the experiment group after intervention was 4.45%,being significantly lower than 13.12% before intervention (χ2 =188.293,P <0.001).While the incidence rate of non -fatal drowning in the control group after intervention was 7.47%, having no significant difference with 6.90% before intervention (χ2 =0.896,P =0.344).And the probability of non -fatal drowning for the intervention group was 0.762 time of the control group.Conclusion The model of integrated drowning interventions,based on the ecological approach and initiated by Ningbo,was proven to be effective and worth popularizing.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the different effects of Shengmai injection on testicular injury after testis torsion/detorsion in rats of different ages.</p><p><b>METHODS</b>Sixteen healthy male SD rats aged 3, 6 and 12 weeks were equally randomized into an experimental group (testicular torsion/detorsion plus Shengmai injection) and a control group (testicular torsion/detorsion plus saline). The rat models of testicular torsion were killed 24 h after surgery for the measurement of total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the testis.</p><p><b>RESULTS</b>Compared with the controls, the 3- and 6-week-old rats of the experimental group showed no significant changes in T-AOC, SOD activity and MDA content (P > 0.05), while the 12-week-old experimental rats exhibited a remarkable increase in SOD and T-AOC and an obvious decrease in MDA content (P < 0.05).</p><p><b>CONCLUSION</b>Shengmai injection has a protective effect against acute ischemia-reperfusion testicular injury after torsion/detorsion in rats, but the effect varies with the age, more obvious in older ones.</p>